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Boreal Genomics instruments are based on SCODA: a novel, proprietary electrophoresis technology that selectively concentrates nucleic acids from large sample volumes to a small, concentrated amount of gel or buffer.
This is accomplished by means of rotating electric fields that act uniquely on long, charged polymers and leave other molecules unaffected. The electric field parameters can be adjusted to select very specifically for nucleic acids, rejecting contaminants that, in affinity-based purification methods, often co-elute with DNA and RNA.
As an example, SCODA can reject humic acids 100 to 1000-fold more effectively than competing bead-and column-based preparation methods. Protein rejection from E. coli lysate is 70-fold better than standard columns.
The method allows for very simple operation: 1 to 5 mL of sample is pipetted into a sample chamber next to the gel, the gel cassette is inserted in the instrument, and, following automated injection and concentration, the purified product is recovered by pipetting 10 to 60 μL of buffer from a central reservoir.
The purification protocol can include electrophoretic washing – application of electric fields that remove contaminants from the gel while locking the DNA product in the center to increase rejection of contaminants from unusually dirty samples.
The SCODA technology is fundamentally different from other electrophoretic preparation methods, and confers some unique advantages:
- Exceptional contaminant rejection
- High yield even in very dilute samples
- Simple operation
- Protection of high molecular weight targets
For contaminated, dilute or otherwise difficult samples, SCODA technology opens the door to success.
For additional information please read our latest technical presentation and white papers.
SCODA Technical Presentation.
White Paper - Plant material extraction.
White Paper - Low abundance extraction.
White Paper - Contaminant rejection.
or contact info@borealgenomics.com.
SCODA stained DNA focusing video.
