OnTarget™ is a powerful new tool for detection and quantification of tumor mutations in plasma or tissue.

 

  • Detect up to 100 mutations without sample splitting
  • Quantify mutations present at 0.01% allele frequency and above
  • Customize panels for any set of mutations

 

The OnTarget system is available via commercial partnership for clinical and pharmaceutical services.

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The OnTarget Workflow

Mutation enrichment with OnTarget™ adds only approximately one hour to a standard next-generation sequencing workflow.

 

Quantify

DNA extracted and quantified with qPCR

Barcode

Limited-cycle PCR with sample-ID barcodes

Select Mutations

Mutant alleles enriched over wild-type with the OnTarget instrument

Construct Library

Secondary indexing barcodes added and samples pooled

Sequence

Mutations detected using the MiSeq

Analyze

Mutations quantified with the OnTarget data analysis software

 


 

OnTarget Instrument

 

OnTarget™ Mutation Detection is based on targeted analysis of mutant alleles over wild-type nucleic acids in an optimized sample-to-answer workflow with single molecule sensitivity.

The OnTarget instrument uses Boreal’s highly selective molecular separation technology to enrich targeted alleles – learn more about the OnTarget technology.

Boreal has developed a combined OnTarget + MiSeq workflow to optimize sample and mutation multiplexing; however, OnTarget can also be integrated with other mutation detection assays.

View the system specification

 


 

Mutation Detection Kits

 

OnTarget™ reagent kits include all materials necessary for pre-amplification, enrichment, and library preparation for the MiSeq.

 


 

Data Analysis Software

 

The OnTarget system includes a data analysis tool to process MiSeq FASTQ files and generate a report with mutations detected, allele quantification, and sample-specific assay LOD.

 


 

System Specification

The OnTarget assay has no minimum volume or input mass requirement. Boreal provides the following recommended sample guidelines

Performance

  • Single molecule sensitivity with 0.01% specificity
  • ≥ 0.01% mutant allele frequency

Recommended Inputs

  • Plasma ≥ 1 mL
  • FFPE Tissue ≥ one 5 µm section (5 mg)
  • Fresh-Frozen Tissue ≥ 1 mg
  • Purified DNA no minimum input mass – number of genome equivalents will determine final assay sensitivity

 


 

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