OnTarget™ is a powerful new tool for detection and quantification of tumor mutations in plasma or tissue.
- Detect up to 100 mutations without sample splitting
- Quantify mutations present at 0.01% allele frequency and above
- Customize panels for any set of mutations
The OnTarget system is available via commercial partnership for clinical and pharmaceutical services.
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The OnTarget Workflow
Mutation enrichment with OnTarget™ adds only approximately one hour to a standard next-generation sequencing workflow.
DNA extracted and quantified with qPCR
Limited-cycle PCR with sample-ID barcodes
Mutant alleles enriched over wild-type with the OnTarget instrument
Secondary indexing barcodes added and samples pooled
Mutations detected using the MiSeq
Mutations quantified with the OnTarget data analysis software
OnTarget™ Mutation Detection is based on targeted analysis of mutant alleles over wild-type nucleic acids in an optimized sample-to-answer workflow with single molecule sensitivity.
The OnTarget instrument uses Boreal’s highly selective molecular separation technology to enrich targeted alleles – learn more about the OnTarget technology.
Boreal has developed a combined OnTarget + MiSeq workflow to optimize sample and mutation multiplexing; however, OnTarget can also be integrated with other mutation detection assays.
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Mutation Detection Kits
OnTarget™ reagent kits include all materials necessary for pre-amplification, enrichment, and library preparation for the MiSeq.
Data Analysis Software
The OnTarget system includes a data analysis tool to process MiSeq FASTQ files and generate a report with mutations detected, allele quantification, and sample-specific assay LOD.
The OnTarget assay has no minimum volume or input mass requirement. Boreal provides the following recommended sample guidelines
- Single molecule sensitivity with 0.01% specificity
- ≥ 0.01% mutant allele frequency
- Plasma ≥ 1 mL
- FFPE Tissue ≥ one 5 µm section (5 mg)
- Fresh-Frozen Tissue ≥ 1 mg
- Purified DNA no minimum input mass – number of genome equivalents will determine final assay sensitivity
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