Complete our sample submission form containing shipment instructions
3. Mutation Detection
Boreal analyzes samples with the OnTarget assay
4. Receive Report
Boreal provides a detailed report including measured allele frequency
The OnTarget workflow takes only two days from sample to sequencing results; we will provide aquote for current turnaround upon acceptance of the sample submission form and statement of work.
An OnTarget Mutation Analysis report includes summary of mutations detected with the assay, measured allele frequency, and limit of detection for each mutation in the panel. The report also includes a detailed analysis of sample QC, summary of methods and data analysis, and complete analysis of individual allele detected/not detected results.
Request a sample submission form containing shipment and packaging instructions to define samples for mutation profiling.
The OnTarget assay has no minimum volume or input mass requirement. Boreal provides the following recommended guidelines for sample submission – samples outside of these ranges can be accepted with an approved statement of work.
Single molecule sensitivity with 0.01% specificity
≥ 0.01% mutant allele frequency
Plasma ≥ 1 mL
FFPE Tissue ≥ one 5 µm section (5 mg)
Fresh-Frozen Tissue ≥ 1 mg
Purified DNA no minimum input mass – number of genome equivalents will determine final assay sensitivity