Boreal’s technology is unmatched in its ability to provide highly purified DNA up to 1 Mb in length from environmental samples, ideal for the construction of large-insert metagenomic libraries.
- Extract intact DNA from 30 kb to 1 Mb in length from environmental samples
- Recover up to 100 ug of highly purified DNA directly from crude extracts of up to 1.5 g of soil
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Highest Molecular Weight Recovery
This Ultra-high molecular weight (Ultra-HMW) DNA extraction protocol purifies and concentrates intact DNA up to 1 Mb in length from soil samples, while avoiding the shearing forces associated with mechanical lysis and columns. A combination of exceptional inhibitor removal, minimal losses, and non-mechanical purification enables recovery of high yield and purity DNA directly from crude extracts of up to 1.5 g of soil, and similar environmental samples.
Minimal Hands-on Processing
Recovery of up to 100 ug of highly purified DNA is achieved by the Aurora instrument; an otherwise unattainable result for all but the most involved manual extraction methods. The ease of use of the system minimizes protocol hands-on time, automating nucleic acid purification and concentration.
Ready for Downstream Analysis
The DNA recovered is ideally suited for applications requiring Ultra-HMW from environmental samples that contain high levels of contaminants and inhibitors of enzymatic activity, including the construction of large-insert metagenomic libraries. This protocol can also be adapted to purify and concentrate Ultra-HMW DNA from agarose plugs as an alternative to agarase digestion or dialysis.
Specifications
| Parameter | Specification |
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| Input | 1.5 g |
| Process Time: Modified Liles lysis | 2 days |
| Run Time: Aurora Instrument | 15 minutes (hands-on) 30 hours (instrument) |
| Expected Molecular Weight Recovery | 30 kb – 1 Mb |
| Capacity | 100 ug |
| Output Volume | 60 mg LMP agarose plug |
| Buffer | 2x TBE, pH 6 – 8 |
References
- Isolation and Cloning of High-Molecular-Weight Metagenomic DNA from Soil Microorganisms. Liles et al. Cold Spring Harbor Protocols; 2009: pdb.prot5271
- Ultra-High Molecular Weight DNA Soil Protocol for Aurora Instruments. May2011.
