Boreal’s technology isolates and purifies DNA ready for obtaining Short Tandem Repeat (STR) profiles from challenging samples including trace blood collections, large volume surface swabs, touch samples, bone and hard tissues, environmental samples, and others.
- Efficient removal of humic acid, tannic acid, melanin, hematin, calcium, silica and other inhibitors
- Minimal loss of DNA during sample purification
 Identifiler® Plus results representing peak height RFU averages for QIAquick® and SCODA purification methods of spiked samples |
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Highest Yield and Purity
Boreal’s technology can be used to purify forensic samples as an alternative to other methods or when other techniques fail to remove all impurities. SCODA technology removes PCR inhibitors such as humic acid, tannic acid, melanin, hematin, calcium, silica much more effectively than silica-membrane columns or phenol-chloroform extraction.
Concentrated and ready for SpeedVac processing or direct PCR analysis
A combination of effective removal of inhibitors, low losses and harvesting of the final product in a small volume of aqueous buffer, enables the forensic DNA to be directly used for PCR analysis. If further concentration is needed, the exceptional removal of inhibitors makes it possible to perform SpeedVac analysis without compromising DNA purity.
Specifications
| Parameter | Specification |
|---|---|
| Input | Process forensic sample in <5ml buffer |
| Run Time: Aurora Instrument | 15 minutes (hands-on) 4 hours (instrument) |
| Yield | >60% of input |
| Output Volume | 60 uL |
| Buffer | 2x TBE, pH 6 – 8 |
References
- The Use of Synchronous Coefficient of Drag Alteration (SCODA) Technology to Extract, Purify, and Concentrate DNA from Challenging or Degraded Forensic Samples. Sarah Schmedes, Laura Mai, Pam Marshall, Jonathan King, Andre Marziali, and Bruce Budowle (2011). Twenty Second Promega International Symposium on Human Identification.
