Boreal’s technology can be applied universally to samples from diverse sources, rescuing inhibited samples and purifying crude lysates to produce high purity nucleic acids for any downstream application
- Remove inhibitors that remain despite purification by other methods
- Recover DNA directly from crude lysates
- Purify contaminants and enzymatic inhibitors regardless of species
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Rescue Your Inhibited Samples
Boreal’s technology simultaneously purifies and concentrates nucleic acids from preparations that remain inhibited despite extraction by other methods. A combination of exceptional contaminant removal and low losses make this protocol ideal for clean-up of any sample that has already been lysed and partially, yet insufficiently, purified.
Recover DNA from Dilute Crude Lysates
The Aurora instrument works directly with small volumes of crude lysate, even those containing only trace amounts of nucleic acids, by efficiently re-concentrating nucleic acids following dilution to the 5 mL input volume.
Contaminant-independent Removal
The SCODA technology removes contaminants and enzymatic inhibitors regardless of species, offering a truly generic method for nucleic acid clean-up, unlike other technologies designed for a specific class of contaminant molecules.
Specifications
| Parameter | Specification |
|---|---|
| Input | Output of commercial nucleic acid extraction kit, or Crude lysate diluted to 5 mL and below 100 uS/cm |
| Run Time: Aurora Instrument | 2 – 3 minutes (hands-on) 1 – 4 hours (instrument) |
| Expected Molecular Weight Recovery | 300 bp / 500 nt – 50 kb |
| Capacity | 100 ug |
| Output Volume | 60 uL |
| Buffer | 0.25x TBE, pH 6 – 8 |
References
- Pel J, Broemeling D, Mai L, Poon HL, Tropini G, Warren RL, Holt RA, and Marziali A “Non-linear Electrophoretic Response Yields a Unique Parameter for Separation of Biomolecules” PNAS, 2009 September 1; 106(35): 14796-14801.
- Nucleic Acid Clean-up Protocol for Aurora Instruments. BG-106-0001-AA-D, November 2010.
