Boreal’s technology is unmatched in its ability to provide highly purified high molecular weight DNA from stool samples, outperforming alternate methods in yield, purity, and molecular weight recovery.
- Intact fragments up to 50 kb without mechanical lysis or columns
- Recovery of high yield and purity nucleic acids directly from crude extracts of up to 0.2 g of stool
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Highest Yield and Purity
This high molecular weight (HMW) DNA extraction protocol concentrates and purifies intact fragments up to 50 kb directly from stool samples without mechanical lysis or columns. A combination of exceptional removal of inhibitors, low losses, and non-mechanical purification enables recovery of high yield and purity nucleic acids directly from crude extracts of up to 0.2 g of stool or similar highly inhibited samples
Highest Molecular Weight Recovery
Recovery of DNA up to 50 kb is optimized in this protocol, enabling extraction of larger intact fragments than commercial kits in a repeatable process that minimizes manual sample processing.
Specifications
| Parameter | Specification |
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| Input | 0.2 g |
| Process Time: Modified Zhou Lysis | 5 – 6 hours |
| Run Time: Aurora Instrument | 2 – 3 minutes (hands-on) 4 hours (instrument) |
| Expected Molecular Weight Recovery | 20 – 50 kb |
| Capacity | 100 ug |
| Output Volume | 60 uL |
| Buffer | 0.25x TBE, pH 6 – 8 |
References
- DNA recovery from soils of diverse composition. Zhou J, Bruns MA, Tiedje JM. Appl Environ Microbiol. 1996 Feb; 62(2):316-22
- High Molecular Weight DNA Stool Protocol for Aurora Instruments. January 2011.
