Detect and monitor tumor mutations in plasma
What is OnTarget?
Boreal’s OnTarget™ is a highly sensitive mutation detection platform that enables accurate tumor profiling and real-time monitoring of tumor evolution from cell-free DNA in plasma.
The OnTarget™ assay reveals tumor mutations that are undetectable with other methods to help guide selection of therapy, improve clinical trial efficiency, and accelerate drug development programs.
How OnTarget Works
OnTarget™ mutation detection is based on targeted analysis of mutant alleles over wild-type DNA in an optimized sample-to-answer workflow that provides single molecule sensitivity.
Quantify
DNA extracted and quantified with qPCR
Barcode
Limited-cycle PCR with sample-ID barcodes
Select Mutations
Mutant alleles enriched over wild-type with the OnTarget™ instrument
Construct Library
Secondary indexing barcodes added and samples pooled
Sequence
Mutations sequenced using the MiSeq
Analyze
Mutations quantified with the OnTarget™ data analysis software
The OnTarget Assay
OnTarget™ mutation detection is available in two convenient formats:
How to Order OnTarget
Services
Complete our sample submission form for research-use OnTarget™ mutation detection services.
System
Contact our OnTarget™ sales team to discuss optimal system configuration including instrument, reagent kits, and data analysis software.
Panels
Boreal offers pre-configured or customizable mutation panels - contact our applications team to discuss custom OnTarget™ panel development.